dye labelled monoclonal antibody assay for detection of toxic shock syndrome toxin -1 from staphylococcus aureus

Authors

v javid khojasteh department of bacteriology, guilan university of medical sciences, faculty of shahid beheshty, rasht, iran.

ha foster center of parasitology and disease reserch, school of environment and life sciences, university of salford, the crescent, salford m5 4wt, uk.

abstract

objective : the aim of study was to develop a rapid assay, dye labelled monoclonal antibody assay (dlmaa), using non-radioactive organic synthetic dyes for identification of toxic shock syndrome toxin-1 (tsst-1) producing strains of staphylococcus aureus. materials and methods: the assay protocol required only two simple steps; addition of tsst-1 antigen to a nitrocellulose membrane and then adding a colloidal dye labelled antibody (d/a) suspension detection reagent. results : the sensitivity and specificity of the assay was determined relative to positive and negative strains compared to an elisa assay. overall 100% agreement was found between both assays. the sensitivity for detection of tsst-1 was 30 ng. conclusion : the dlmaa did not require handling and disposal of radioactive materials. it is a rapid qualitative technique for detection of tsst-1 toxin at room temperature within a short time.

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Journal title:
iranian journal of microbiology

جلد ۳، شماره ۴، صفحات ۱۷۰-۱۷۶

Keywords
[ ' m o n o c l o n a l a n t i b o d y ' , ' s t a p h y l o c o c c u s a u r e u s ' , ' t s s t ' , 1 , ' c o l l o i d a l d y e ' , ' e l i s a ' ]

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